These steps include designing the optimal gRNA based on computational and mathematical predictions and the application of functional experimental off-target cleavage validation assays using GUIDE-seq8 and rhAmp-Seq10 technologies. therapy. Visual Abstract Open in a separate window Introduction Multivirus-specific T cells (VSTs) have proven safe and effective for the treatment of life-threatening viral infections such as … Continue reading These steps include designing the optimal gRNA based on computational and mathematical predictions and the application of functional experimental off-target cleavage validation assays using GUIDE-seq8 and rhAmp-Seq10 technologies